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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Robust and Reproducible Monoclonal Antibody Production Using a Transgenic Silkworm System
doi: 10.3390/ijms262110287
Figure Lengend Snippet: Process analyses of three batches of pilot-scale purification involving extraction (EXT), Protein A chromatography (ProA), ceramic hydroxyapatite chromatography (CHT), multimodal anion exchange chromatography (CA), and tangential flow filtration (TFF). ( A ) Trends in the recovery rates of 1C10K for each purification process. ( B ) SDS-PAGE under reducing conditions for each purification step in batch B. HC and LC indicate heavy and light chains of IgG, respectively. ( C ) Residual host cocoon proteins (HCP) levels, measured by an HCP assay. Asterisks indicate values below the lower limit of quantitation, and in such cases, the height of the bars represents those limits. ( D ) Size exclusion chromatography (SEC) chromatograms of each purification step in batch B. Black arrow indicates citric acid, a component of the sample solution. Inset shows a detailed view of elution peaks of the oligomeric IgGs. ( E ) Ratio of residual oligomeric IgGs measured by SEC.
Article Snippet: The resultant 1C10K solution was loaded onto a ceramic hydroxyapatite chromatography column packed with 5 L of
Techniques: Purification, Extraction, Chromatography, Filtration, SDS Page, Quantitation Assay, Size-exclusion Chromatography